Biochemical identification of unknown bacteria Lab Report

Biochemical identification of unknown bacteria - science lab Report Example1)Nutrient agar slantGiven culture was streak on the nutrient agar slant and incubated at two different temperature 25C and 37C respectively. aft(prenominal) 24h of incubation, slants were taken out from incubator and growth was observed. Growth on tube incubated at 37C was found to be effuse and the growth was extensive while in case of tube incubated at 25C growth was found to be lesser compared to previous tube and echinulated in nature. on that point was no pigmentation observed and growth was found to be whitish in color and translucent in nature. Results indicated that given unknown bacteria is either Escherichia coli, Enterobacter aerogenes or Staphylococcus aureus ,being human /animal inhabitant The best temperature for growth of these bacteria is 37C (i.e. human body temperature) while in case of genus Pseudomonas aerogenosa being free living as well as human inhabitant, it can grow at 25C as wel l as 37C . Similarly it also produces drear green pigment and grows as mucoid dependence.2)Nutrient agarGiven culture was streak on to the nutrient agar plate using quadrant streak technique and incubated at 37C for 24 h. After incubation colonies were found to be convex, circular and having entire margin. Optically colonies were found to be translucent and without any pigmentation. This observation again ruled out the possibility of Pseudomonas aeroginosa. Similarly colony of Staphylococcus aureus is opaque, slightly elevated, appears yellowish white.